Three-phase partitioning (TPP) is an efficient and cost-effective method for protein purification that exploits the simultaneous addition of salts and organic solvents to an aqueous protein extract.

Affinity chromatography has emerged as an indispensable tool in modern protein purification, leveraging highly selective interactions between target proteins and immobilised ligands. This technique, ...

The purification of proteins and antibodies is a critical process in biotechnology and pharmaceutical industries. Traditional methods often involve single-use materials, leading to high costs and ...

Today, crystallization is used primarily to analyze the structures of proteins. It also is gaining favor as a purification method for elastase, proteases, albumin, and lipase, in addition to ...

Researchers at Queen’s University in Kingston, Ontario, Canada, are developing an alternative to nickel-nitrilotriacetic acid (Ni-NTA) to purify recombinant proteins. Because it uses common ...

Protein yield and ease of purification are two important criteria in choosing a system for the expression/ purification of recombinant proteins. The IMPACT (Intein Mediated Purification with an ...