Total internal reflection fluorescence (usually abbreviated as TIRF) microscopy is an imaging method that illuminates fluorescence in a shallow area of the specimen in order to enhance the signal ...

The DeltaVision OMX V4 enables Total Internal Reflection Fluorescence Microscopy (TIRF). This technique uses an evanescent wave to selectively excite fluorophores that are close to the coverslip. This ...

Total internal reflection inside a 90 o glass prism The critical angle for the type of glass used to make the prism = 42 o. The ray of light is turned through 180 o.

David N. Bowser, Baljit S. Khakh, Two Forms of Single-Vesicle Astrocyte Exocytosis Imaged with Total Internal Reflection Fluorescence Microscopy, Proceedings of the National Academy of Sciences of the ...