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Wavelength Accuracy Calibration in HPLC 1. Purpose Wavelength accuracy calibration ensures that the detector (usually a UV-Vis detector) is measuring light at the correct wavelength. Accurate wavelength measurement is critical because absorbance values depend on precise wavelength alignment with analyte absorption maxima. 2. Principle Certain reference materials (filters or solutions) have well-defined absorbance peaks at specific wavelengths. By scanning these standards, the detector’s measured
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