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6:44
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The Nomadic Scientist
Sandwich ELISA Principle and Applications.
A sandwich ELISA measures antigen between two layers of antibodies (capture antibody & detection antibody). The target antigen must contain at least two antigenic sites capable of binding to antibodies. Advantage of Sandwich ELISA. 1. Use for quantifying antigens. 2. Sandwich ELISA is about 2 to 5 times more sensitive than direct or indirect ...
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